GAD2 and diabetes mellitus: In order to determine in the literature (26) addressed effect of FBS on antigen-specific tolerance induction by tolDCs as well as to reflect the fact that DCs for human trials are prepared in SF conditions, we have compared the diabetes-preventive capacity of unloaded tolDCs cultured in serum-supplemented vs. SF conditions as well as retested effects of tolDCs-GAD65 and tolDCs-OVA in SF conditions (Figure 3A).