Based on these qPCR analyses, the mRNAs confirmed to be decreased in ovarian cancer ascites compared to peritoneal fluid EVs included LAMA4, CA11, and MEDAG. EVs from a high grade serous ovarian adenocarcinoma cell line OVCA3 also contained less abundance of CA11 compared to controls from immortalized epithelial fallopian and ovarian cells. The gene discussed is MEDAG; the disease is ovarian serous adenocarcinoma.