Based on these qPCR analyses, the mRNAs confirmed to be decreased in ovarian cancer ascites compared to peritoneal fluid EVs included LAMA4, CA11, and MEDAG. EVs from a high grade serous ovarian adenocarcinoma cell line OVCA3 also contained less abundance of CA11 compared to controls from immortalized epithelial fallopian and ovarian cells. Here, LAMA4 is linked to ovarian cancer.