In a large autosomal-dominant PPCD-affected family of Czech origin (C15, Figure 1A), targeted Sanger sequencing did not identify any likely disease-associated variants within established PPCD-associated genes, including the OVOL2 promoter region.11, 23 Furthermore, quantitative real-time PCR and Illumina HumanOmniExpress BeadChip SNP array analysis did not detect CNVs encompassing known PPCD-associated genes.10 The gene discussed is OVOL2; the disease is posterior polymorphous corneal dystrophy.