Given that the c.20+544G>T variant lies within a regulatory region of GRHL2, a 2,728-bp region encompassing the 5′ UTR, exon 1, and partial intron 1 of GRHL2 containing predicted regulatory regions and transcription factor binding sites (Figure S2), was therefore PCR amplified and Sanger sequenced in unsolved PPCD cases. This evidence concerns the gene GRHL2 and posterior polymorphous corneal dystrophy.