However, despite the finding that the camalexin biosynthesis genes CYP71A12, CYP71A13 and PAD3 were up‐regulated at 24 hpi, no significant change in the transient transformation efficiency could be observed in these mutants relative to Col‐0 (Fig. 3), probably because of the low levels of camalexin produced in seedlings at the early stage of infection (Fig. 8a). Here, PADI3 is linked to infection.