Apart from G0/G1 arrest, piperine arrests osteosarcoma cells at G2/M phase of cell cycle through the downregulation of cyclin B1 and enhanced phosphorylation of cyclin-dependent kinase-1 (CDK1) and checkpoint kinase 2 (Chk2) (Zhang et al., 2015). This evidence concerns the gene CHEK2 and osteosarcoma.