DR3 (Apo3, TRAMP, LARD, WSL-1, TNFRSF25) is predominantly expressed by T lymphocytes and to a much less extent by other types of cells.30,31 Human colorectal cancer cell line HT29 is one of the cancer cell lines that have undetectable DR3 expression.32 HT29 cells were reported to be resistant to taxol and a small-molecule inhibitor of Eg5 (also a spindle poison),33 and our data was consistent with this observation when HT29 cells were treated with diazonamide or taxol for 48 h (Supplementary information, Figure S4A). Here, TNFRSF25 is linked to cancer.