ACTA1 and Hepatic fibrosis: Immunofluorescence and qRT-PCR were employed to quantify the expression of hepatic-fibrosis-related mRNA, including α-SMA, ACTA2, COL1A, LOX and LOXL2. The results showed that VPA effectively reversed hHSCs activation and played an important role in anti-hepatic fibrosis, and successfully established a quiescence hHSCs model, which provided the conditions for downstream screening of anti-fibrosis associated lncRNA in hHSCs.