Having demonstrated the potential application of hydrofection to treat haemophilia B, Matsui et al. [36] employed this strategy to test its possible use in haemophilia A. Accordingly, these authors transferred the entire human FVIII cDNA in a piggyback transposon to mice and reported protein plasma expression (40 mU/mL) for more than 300 days, with functional activity in FVIII knockout mice. The gene discussed is F8; the disease is hemophilia A.