The objectives of this study were therefore (1) to synthesize and characterize Lf-bearing DAB dendrimer, (2) to assess the transfection and anti-proliferative efficacy of the DAB-Lf dendriplexes encoding TNFα, TRAIL, or IL-12, on prostate cancer cells in vitro, and (3) to evaluate their therapeutic efficacy in vivo, following intravenous administration to mice bearing prostate tumors. This evidence concerns the gene TNF and prostate neoplasm.