The objectives of this study were therefore (1) to synthesize and characterize Lf-bearing DAB dendrimer, (2) to assess the transfection and anti-proliferative efficacy of the DAB-Lf dendriplexes encoding TNFα, TRAIL, or IL-12, on prostate cancer cells in vitro, and (3) to evaluate their therapeutic efficacy in vivo, following intravenous administration to mice bearing prostate tumors. The gene discussed is TNF; the disease is prostate carcinoma.