No difference was instead detected between occurrence of gene amplification and BRAF mutation status both in vitro (see Tables 1 and 2) and in vivo (data not shown): 15/20 (75%) BRAF-mutated and 9/12 (75%) BRAF-wild-type cell lines as well as 57/576 (9.9%) BRAF-mutated and 59/683 (8.6%) BRAF-wild-type melanoma tissues were found to carry amplification in at least one gene locus. The gene discussed is BRAF; the disease is melanoma.