Interphase melanoma cell lines showed a similar high level of amplification for EGFR gene (12/32; 37.5%) (Table 2; Supplementary Table 1); again, cell lines derived from primary melanoma (2/8; 25%) presented a significantly lower level of gene amplification compared to those derived from metastatic melanoma (10/24; 41.7%) (p = 0.041; Chi-square). Here, EGFR is linked to metastatic melanoma.