Tumor size, depth of tumor invasion, lymph node metastasis, stage, PTS, and serum concentrations of CEA and CA19-9 all correlated significantly with prognosis in univariate analysis (P = 0.0032, P < 0.0001, P = 0.0003, P < 0.0001, P < 0.0001, P = 0.0338, and P = 0.0043, respectively) (Table 3). This evidence concerns the gene CEACAM5 and metastatic malignant neoplasm in the lymph nodes.