Validation by qRT-PCR of the selected gene targets (CYTH4, GIMAP4, HPGD, NFKBIA, PLAUR, TNFAIP3, TNFSF14, and VEGFA) confirmed the RNA-seq results, when tested in DCs from 10 patients with T1D (Figure 6). This evidence concerns the gene VEGFA and type 1 diabetes mellitus.