To evaluate to what extent the absence of IFN-α/β signaling in astrocytes predisposes astrocytes over other cell types to enhanced infection, brains from infected mGFAPcre IFNARfl/fl and IFNARfl/fl mice were analyzed for viral N protein in combination with antibodies (Abs) specific for neurons (NeuN), astrocytes (glial fibrillary acidic protein [GFAP]), and microglia/macrophages (IBA1). This evidence concerns the gene GFAP and infection.