A NGS-identified variant in PPP1R3B gene (rs61756425 G/T p.S41R) emerged as more frequent in NAFLD patients than in controls (χ2 = 16.11, P < 0.001) and as the strongest independent genetic predictor of severe hepatic steatosis (OR = 32.6, 95% CI, 4.22-251.4, Padj = 0.001). The gene discussed is PPP1R3B; the disease is metabolic dysfunction-associated steatotic liver disease.