APC, MGMT, SEPT9 and RASSF1A methylation levels were significantly higher in CRC compared to normal tissues (P = 0.005, P < 0.001, P < 0.001 and P = 0.002, respectively), whereas IGF2 methylation levels were significantly lower in CRC (P = 0.025), as expected (Fig. 1). This evidence concerns the gene RASSF1 and colorectal carcinoma.