In hGFAP-Cre;Nf1±;p53fl/fl;Ptenfl/+ glioma GEM model (100% penetrance in 20–40 weeks), temozolomide could diminish the proliferating GBM cells, while repopulation of GBM was driven by residual quiescent GICs, recruited (to expand and/or divide) after temozolomide was discontinued (53). The gene discussed is HGFAC; the disease is glioblastoma.