To study further the role of FOXO6 in breast cancer, first we used qRT-PCR to examine the expression of FOXO genes in a series of 39 human breast cell lines (including seven normal breast cell lines (N) and thirty-two tumorigenic breast cell lines (T), Supplementary Table 2) and in a large series of 527 human breast tumours (clinical parameters presented in Supplementary Table 3). This evidence concerns the gene FOXO6 and breast neoplasm.