To determine the effects of SALL1 inhibition on cellular proliferation and leukemia engraftment in mice, HEK293 cells, AML cell lines MV4-11 and THP1cells were stably infected with either sh-RNA constructs (NO 4) directed against SALL1 (sh-SALL1) or against GFP (ctrl-GFP-SALL1) with over 80% transfection efficiency. The gene discussed is SALL1; the disease is leukemia.