To exclude the effects of initial weight differences between p533KR/3KR/mdmx-/- and p533KR/3KR mice on the outcome of diet-induced obesity, bodyweight-matched p533KR/3KR/mdmx-/- and p533KR/3KR mice were treated with HFD. Here, MDM4 is linked to obesity due to melanocortin 4 receptor deficiency.