After high fat diet treatment, there was apparent accumulation of lipid droplets in the liver (hepatic steatosis) from p533KR/3KR mice from the histopathological analysis, in contrast, the hepatic steatosis in p533KR/3KR/mdmx-/- mice was largely prevented indicated by significantly reduced lipid droplets (Figure 3F ii vs. i). This evidence concerns the gene MDM4 and Hepatic steatosis.