To understand the molecular basis of the miR-532-5p’s tumor-suppressing effects, we performed luciferase reporter assays to assess the effect of miR-532-5p on the NF-κB, MAPK, and Wnt signaling pathways, which are well-known positive regulators of tumor angiogenesis and metastasis. This evidence concerns the gene NFKB1 and neoplasm.