As SMAD1/5/8 phosphorylation, due to iron restriction and anaemia, was not induced 14 days after heat-killed BA administration, we administered the vital bacterium S. aureus in another set of experiments to mice with and without hepatocyte-specific Alk3 deficiency and analysed the protein phosphorylation 24 h later. The gene discussed is SMAD1; the disease is anemia (phenotype).