Our treatment with AqE.NS also shows a gradual return to euglycemia, followed by an increase in insulin in sera, in agreement with the results of Kanter et al. [36] who induced diabetes by streptozotocin (STZ) and those of Rchid et al. [37] and Benhaddou-Andaloussi et al. [38] who demonstrated in vitro that N. sativa extract improved β cell proliferation and increased insulin secretion stimulated by glucose, consequently attenuating the progression of diabetes. This evidence concerns the gene INS and diabetes mellitus.