BRAF and neoplasm: The pre-screen procedure of mutation analysis indeed increased sample size of BRAF mutated samples in our FFPE dataset and facilitate an accurate estimation of sensitivity, but because the pre-screen procedure ranked tumors with scores without knowing which tumor is BRAF mutated, it also increased the sample size of BRAF wild-type tumors that are true BRAF mutation-like subtype in these 302 FFPE samples, which resulted in a biased estimation of reduced specificity.