Immortalized human astrocytes transduced with D463H mutant PRKCA alongside a series of malignant glioma cell lines harboring wildtype PRKCA alleles were grown in soft agar containing either DMSO vehicle or 5 μM trametinib, a small molecule inhibitor of MEK that is FDA-approved for the treatment of melanoma. Here, PRKCA is linked to malignant glioma.