Analyses of PBMC from multiple subjects showed strong statistical significance between whole and CD8-depleted PBMC (p = 0.0006) (Fig 8B); in contrast, depletion of CD8 T cells in Ad5 vector-stimulated PBMC (HVTN204) had no significant impact on HIV infection rate of Ad5 vector-specific CD4 T cells (Fig 8A and 8B). The gene discussed is CD4; the disease is HIV infectious disease.