Then, lactoferrin (Lf) was linked with Fe3O4 NPs by the amide linkage between amino groups on Lf and the carboxyl groups of PEG-coated Fe3O4 NPs, which could be bonded on the surface of receptors overexpressing glioma cells, and as a targeted ligand to further improve the targeting properties of the nanocomposites. This evidence concerns the gene LTF and glioma.