Finally, since more recently, non-canonical autophagy, also defined as LAP, has been extensively described for infection with A. fumigatus (29, 31), we performed shRNA of the main key players (Rubicon, Nox2, Atg7) of LAP on D1 cells upon Aspergillus treatment and we went to analyze IL-2 transcription as a key response downstream of NFAT translocation (Figure 4E; Figure S2 in Supplementary Material). The gene discussed is RUBCN; the disease is infection.