To further test PIM1 overexpression in kidney cancer, we utilised a panel of four human ccRCC cell lines (ACHN, 786-O, 769-P and OS-RC-2) and HK-2 cells (an immortalised proximal tubule epithelial cell line) to examine relative PIM1 mRNA expression by qRT-PCR. The gene discussed is PIM1; the disease is kidney cancer.