NFIX suppresses cancer progression in esophageal squamous cell carcinoma (ESCC) and Murine subventricular zone (SVZ) neurogenic niche.23, 24 To investigate the effects of NFIX in HCC cells, we constructed a pNFIX plasmid to express NFIX fused with Flag tag and a pshR-NFIX plasmid to silence NFIX expression. Here, NFIX is linked to esophageal squamous cell carcinoma.