As conflicting results have been reported on the prognostic value of CK20-positive cells in pN0 CRC [9, 10], we used a non-biotin polymer detection system to detect CK20-positive cells in the LNs of pN0 CRC patients and found that 29 out of 56 (52%) LN specimens had CK20-positive cells (Range: 1–35) [11]. This evidence concerns the gene KRT20 and colorectal carcinoma.