Because Th17 cells are predominantly involved in recall responses during OPC (36), we next investigated the role of Casz1 during recall responses to Candida. Analysis of SPLN, LN, and tongue infiltrates at 2 days after secondary infection revealed that the frequency of IL17A producing CD4+ T cells was significantly reduced in Casz1 knockout mice (Figures 5C,D). This evidence concerns the gene CD4 and infection.