In agreement with these results, we found in the present study that Rg1 down-regulated the RhoA/ROCK signal pathway, manifested by the decrease in the phosphorylation of RhoA, MYPT-1, and MLC in myocardium, which was parallel with its attenuating effect on I/R-induced myocardial injury and energy metabolism disorder, highly suggesting RhoA/ROCK signal pathway as a central player in Rg1 action. The gene discussed is PPP1R12A; the disease is Disorder of energy metabolism.