To confirm the performance of dqPCR, a pUC19 plasmid DNA standard and total RNA extracts from A549 lung carcinoma cells were used, and the levels of GAPDH, E-cadherin, N-cadherin, and vimentin at various concentrations were quantified using both qPCR and dqPCR. The gene discussed is CDH1; the disease is lung carcinoma.