C5 and acute kidney injury: We established a rat model of RM-induced AKI through intramuscular glycerol injection into the hind limbs and measured the expression levels of various complement factors: C1q to evaluate the classical pathway; factor B to evaluate the alternative pathway; MBL-A to evaluate the MBL complement pathways; C5a to assess the anaphylatoxic component of the complement system; CD59 to assess complement regulators; and the membrane attack complex.