A novel protocol that integrates fluorescence-activated cell sorting, high-sensitivity single-cell mutation detection and single-cell RNA sequencing of the same single cell was applied to analyze more than 2000 HSCs from patients with CML and to characterize molecular signatures of LSC subpopulations, including BCR-ABL expression, in human CML samples from diagnosis through remission and disease progression [52]. This evidence concerns the gene BCR and chronic myelogenous leukemia, BCR-ABL1 positive.