ZNF660 and pachyonychia congenita: Promoter methylation levels of both genes were similar in AN and BPH samples (Fig. 1A,B; P > 0.1), and ROC curve analysis showed high discrimination between benign (AN and BPH) and PC samples with AUCs of 0.946 and 0.846 for ST6GALNAC3 and ZNF660, respectively (Fig. 1C,D).