During the process of analyzing combination effects of TKI and TOR-KI compounds on expanded B-ALL cells, we created a stromal cell-independent Ph-like cell line (termed TVA1) from a PDX model derived from a bone marrow specimen from a 5-year old female with Ph-like ALL with an ETV6-ABL1 fusion (PAUXZX) treated on the Children’s Oncology Group protocol AALL1131. Here, ABL1 is linked to acute lymphoblastic leukemia.