After confirming which clones did not express miR-21 (Figure 8A), we measured the basal expression of PDCD4 and BTG2 in three PANC-1 KO miR-21 clones (c4, c5 and c6), and compared it with the control pancreatic cancer cell line PANC-1 expressing high levels of miR-21. Here, BTG2 is linked to pancreatic neoplasm.