NUMB and familial pancreatic carcinoma: We measured the expression of key members of the Notch Signaling Pathway (NUMB, DTX4, DTX3L, PSEN1, APH1A, ADAM17 and EP300) in the MiaPaCa-2-miR-148a by qRT-PCR, and compared it with the basal levels of the control pancreatic cancer cell line MiaPaCa-2, expressing very low levels of miR-148a (Figure 7A).