Nested PCR primer pairs generating a 255 bp and a 171 bp fragments for KAI1-WT and KAI1-SP, respectively, were used to screen a small series of eight human breast cancer tissues for the presence of endogenous mRNA for KAI1-WT and KAI1-SP (Figure 1D, lane 1-8) [29]. Here, CD82 is linked to breast carcinoma.