The quantification and statistical evaluation of fluorescence signal intensity was calculated from six independent ROIs as described under Materials & Methods. Moreover, we analysed EGF-R expression as a function of either KAI1-WT or KAI1-SP in human breast cancer cells by Western blot analysis and were able to confirm the results obtained by immunocytochemical analysis (Figure 7B). The gene discussed is EGFR; the disease is breast carcinoma.