Cultured cells derived from metastatic human melanoma such as C8161 and UACC903 show elevated basal levels of activated phospho-ERK [pERK] and phospho-AKT [pAKT] compared to Grm1-negative human primary melanoma line, C81-61, and other normal human melanocytic cell lines [4]. The gene discussed is AKT1; the disease is melanoma.