This is evidenced by direct measurement of intracellular calcium using the Ca2+ binding Fluo-8 AM dye, and the CRAC inhibitor BTP-2 which clearly demonstrated that pevonedistat increased Ca2+ influx in ALL cells, and that activation of p-ERK1/2 was significantly reduced in presence of the Ca2+ chelator BAPTA-AM or the PKC inhibitor enzastaurin. This evidence concerns the gene PRRT2 and acute lymphoblastic leukemia.