In particular, proband 19 carried a p.D2155N (c.6463G>A) variant in TRIO, a gene which has been associated with ASD and ID [50], and proband 18 carried a deletion of five amino acids (c.1029_1043del, p.Ala345_Ala349del) in ARID1A, one of the causal genes for Coffin–Siris syndrome [51]. This evidence concerns the gene TRIO and Coffin-Siris syndrome.