TJP1 and infection: As the barrier function of HIBCPP cells is mainly established via a complex network of TJs similar to other polarized epithelial cells [42, 48], we were interested in the morphology of TJ and AJ following infection with the different E-30 strains, which we analyzed via immunofluorescence analysis of ZO1, occludin, and E-cadherin (Figs. 2, 3, and 4).