Increased expression of EphB4 was confirmed by western blot analysis (Figure 1A) and quantitative real-time RT-PCR (Figure 1B) in A375 melanoma cells transfected with pIRES2-AcGFP1-EphB4 (A375-EphB4), but not in A375 cells transfected with mock plasmid (A375-pIRES) or non-transfected control (A375). The gene discussed is EPHB4; the disease is melanoma.