In this study, to reduce the toxicity and improve the silencing efficiency of the nanoparticle complex, we prepared an FSH peptide-conjugated PEG-PEI copolymer with an increased amount of PEG grafting and encapsulated shRNA to silence the target gene, growth-regulated oncogene α (gro-α), which promotes malignant transformation, tumor growth, and metastatic spread (Yang et al., 2006; Wang et al., 2017). Here, CXCL1 is linked to neoplasm.