Further examining of the 12 polarization markers in each type of microglial cell phenotype showed that nine markers of the M1-type (IL-1β, IL-12, TNF-α, IFN-γ, CCL5, CXCL1, CXCL10, CD16, and CD86) and four markers of the M2-type (IL-1Rα, TGF-β, Arg1, and YM1) were consistently expressed for the entire 12-week period after infection (Figure 3B). This evidence concerns the gene TNF and infection.