MCM7 and keratoconus: In an effort to correlate β-HPV infection with disease pathology during progression of premalignant lesions to KC, we have set up an immunofluorescence protocol that allows both visualization of productive HPV infection in affected skin areas and evaluation of skin homeostasis through detection of the minichromosome maintenance complex component 7 (MCM7), a cellular proliferation marker (Borgogna et al., 2012, 2014).