This is particularly interesting since 48 h after injection into the neural tube, even PKF115–584 pre-conditioned melanoma cells were still positive for the proliferation marker MIB1, and suggests that inhibition of Wnt-signaling with PKF115–584 might lead to both complete inhibition of neural crest migration and invasion (after 48 h) and to a delayed induction of cell death (up to 96 h). The gene discussed is MIB1; the disease is melanoma.