Using a cutoff of greater than 30% increase in viral infection normalized to cell viability in two independent screens, we identified HSPA5, TMED2, SPCS2, and DDIT3 as factors whose overexpression increased DENV infection (Figure 5B and Figure 5—figure supplement 1B), indicating rate limitation associated with these important proviral factors. This evidence concerns the gene TMED2 and viral infectious disease.