In order to verify whether the treatment with the natriuretic peptide affected the Wnt signaling also in these cellular systems as previously demonstrated for other cells (Serafino et al., 2012; Skelton et al., 2013a,b; Vesely, 2013), SHSY5Ywt neuroblastoma cells were treated for 24 h with ANP in a preliminary dose-response experiment (Supplementary Figure S1), for selecting the lowest effective and not toxic dose to be used. This evidence concerns the gene NPPA and neuroblastoma.